Oxysterol-binding protein-related protein 2 is not essential for Phytophthora sojae based on CRISPR/Cas9 deletions.
Identifieur interne : 000707 ( Main/Exploration ); précédent : 000706; suivant : 000708Oxysterol-binding protein-related protein 2 is not essential for Phytophthora sojae based on CRISPR/Cas9 deletions.
Auteurs : Jianqiang Miao [République populaire de Chine] ; Xiuhuan Li [République populaire de Chine] ; Dong Lin [République populaire de Chine] ; Xili Liu [République populaire de Chine] ; Brett M. Tyler [États-Unis]Source :
- Environmental microbiology reports [ 1758-2229 ] ; 2018.
Descripteurs français
- KwdFr :
- Délétion de séquence (MeSH), Gènes essentiels (MeSH), Hydrocarbures fluorés (pharmacologie), Phytophthora (croissance et développement), Phytophthora (effets des médicaments et des substances chimiques), Phytophthora (génétique), Phytophthora (pathogénicité), Pyrazoles (pharmacologie), Récepteurs aux stéroïdes (génétique), Récepteurs aux stéroïdes (physiologie), Systèmes CRISPR-Cas (génétique), Séquence d'acides aminés (MeSH), Virulence (génétique).
- MESH :
- croissance et développement : Phytophthora.
- effets des médicaments et des substances chimiques : Phytophthora.
- génétique : Phytophthora, Récepteurs aux stéroïdes, Systèmes CRISPR-Cas, Virulence.
- pathogénicité : Phytophthora.
- pharmacologie : Hydrocarbures fluorés, Pyrazoles.
- physiologie : Récepteurs aux stéroïdes.
- Délétion de séquence, Gènes essentiels, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), CRISPR-Cas Systems (genetics), Genes, Essential (MeSH), Hydrocarbons, Fluorinated (pharmacology), Phytophthora (drug effects), Phytophthora (genetics), Phytophthora (growth & development), Phytophthora (pathogenicity), Pyrazoles (pharmacology), Receptors, Steroid (genetics), Receptors, Steroid (physiology), Sequence Deletion (MeSH), Virulence (genetics).
- MESH :
- chemical , genetics : Receptors, Steroid.
- chemical , pharmacology : Hydrocarbons, Fluorinated, Pyrazoles.
- drug effects : Phytophthora.
- genetics : CRISPR-Cas Systems, Phytophthora, Virulence.
- growth & development : Phytophthora.
- pathogenicity : Phytophthora.
- chemical , physiology : Receptors, Steroid.
- Amino Acid Sequence, Genes, Essential, Sequence Deletion.
Abstract
Oxysterol-binding protein (OSBP)-related proteins (ORPs) are a large conserved family of lipid transfer proteins in eukaryotes. In oomycetes, some ORPs are the target of the novel oomycide oxathiapiprolin. By searching the Phytophthora sojae genome database, two ORP proteins, PsORP1 (Protein ID: 558498) and PsORP2 (Protein ID: 470921), were found. Here, we investigated the biological function of PsORP2. The expression level of PsORP2 was higher in germinated cysts and late infection than in other developmental stages. However, deletion of PsORP2 using CRISPR/Cas9 had no significant effect on sporangia production, zoospore production, cyst germination, oospore production, virulence or oxathiapiprolin sensitivity. PsORP1 also was not upregulated in ΔPsORP2 transformants. Collectively, our studies demonstrate that PsORP2 is not an essential protein for development or virulence in P. sojae under the conditions we tested.
DOI: 10.1111/1758-2229.12638
PubMed: 29521469
Affiliations:
Links toward previous steps (curation, corpus...)
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Tyler</name><affiliation wicri:level="1"><nlm:affiliation>Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331</wicri:regionArea><wicri:noRegion>97331</wicri:noRegion></affiliation></author></analytic><series><title level="j">Environmental microbiology reports</title><idno type="eISSN">1758-2229</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>CRISPR-Cas Systems (genetics)</term><term>Genes, Essential (MeSH)</term><term>Hydrocarbons, Fluorinated (pharmacology)</term><term>Phytophthora (drug effects)</term><term>Phytophthora (genetics)</term><term>Phytophthora (growth & development)</term><term>Phytophthora (pathogenicity)</term><term>Pyrazoles (pharmacology)</term><term>Receptors, Steroid (genetics)</term><term>Receptors, Steroid (physiology)</term><term>Sequence Deletion (MeSH)</term><term>Virulence (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Délétion de séquence (MeSH)</term><term>Gènes essentiels (MeSH)</term><term>Hydrocarbures fluorés (pharmacologie)</term><term>Phytophthora (croissance et développement)</term><term>Phytophthora (effets des médicaments et des substances chimiques)</term><term>Phytophthora (génétique)</term><term>Phytophthora (pathogénicité)</term><term>Pyrazoles (pharmacologie)</term><term>Récepteurs aux stéroïdes (génétique)</term><term>Récepteurs aux stéroïdes (physiologie)</term><term>Systèmes CRISPR-Cas (génétique)</term><term>Séquence d'acides aminés (MeSH)</term><term>Virulence (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Receptors, Steroid</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Hydrocarbons, Fluorinated</term><term>Pyrazoles</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>CRISPR-Cas Systems</term><term>Phytophthora</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Phytophthora</term><term>Récepteurs aux stéroïdes</term><term>Systèmes CRISPR-Cas</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Phytophthora</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Hydrocarbures fluorés</term><term>Pyrazoles</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Récepteurs aux stéroïdes</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Receptors, Steroid</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Genes, Essential</term><term>Sequence Deletion</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Délétion de séquence</term><term>Gènes essentiels</term><term>Séquence d'acides aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Oxysterol-binding protein (OSBP)-related proteins (ORPs) are a large conserved family of lipid transfer proteins in eukaryotes. In oomycetes, some ORPs are the target of the novel oomycide oxathiapiprolin. By searching the Phytophthora sojae genome database, two ORP proteins, PsORP1 (Protein ID: 558498) and PsORP2 (Protein ID: 470921), were found. Here, we investigated the biological function of PsORP2. The expression level of PsORP2 was higher in germinated cysts and late infection than in other developmental stages. However, deletion of PsORP2 using CRISPR/Cas9 had no significant effect on sporangia production, zoospore production, cyst germination, oospore production, virulence or oxathiapiprolin sensitivity. PsORP1 also was not upregulated in ΔPsORP2 transformants. Collectively, our studies demonstrate that PsORP2 is not an essential protein for development or virulence in P. sojae under the conditions we tested.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">29521469</PMID><DateCompleted><Year>2019</Year><Month>02</Month><Day>25</Day></DateCompleted><DateRevised><Year>2019</Year><Month>02</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1758-2229</ISSN><JournalIssue CitedMedium="Internet"><Volume>10</Volume><Issue>3</Issue><PubDate><Year>2018</Year><Month>06</Month></PubDate></JournalIssue><Title>Environmental microbiology reports</Title><ISOAbbreviation>Environ Microbiol Rep</ISOAbbreviation></Journal><ArticleTitle>Oxysterol-binding protein-related protein 2 is not essential for Phytophthora sojae based on CRISPR/Cas9 deletions.</ArticleTitle><Pagination><MedlinePgn>293-298</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/1758-2229.12638</ELocationID><Abstract><AbstractText>Oxysterol-binding protein (OSBP)-related proteins (ORPs) are a large conserved family of lipid transfer proteins in eukaryotes. In oomycetes, some ORPs are the target of the novel oomycide oxathiapiprolin. By searching the Phytophthora sojae genome database, two ORP proteins, PsORP1 (Protein ID: 558498) and PsORP2 (Protein ID: 470921), were found. Here, we investigated the biological function of PsORP2. The expression level of PsORP2 was higher in germinated cysts and late infection than in other developmental stages. However, deletion of PsORP2 using CRISPR/Cas9 had no significant effect on sporangia production, zoospore production, cyst germination, oospore production, virulence or oxathiapiprolin sensitivity. PsORP1 also was not upregulated in ΔPsORP2 transformants. Collectively, our studies demonstrate that PsORP2 is not an essential protein for development or virulence in P. sojae under the conditions we tested.</AbstractText><CopyrightInformation>© 2018 Society for Applied Microbiology and John Wiley & Sons Ltd.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Miao</LastName><ForeName>Jianqiang</ForeName><Initials>J</Initials><Identifier Source="ORCID">0000-0002-4683-6933</Identifier><AffiliationInfo><Affiliation>Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, 100193, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Xiuhuan</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Pesticides, College of Plant Protection, Shandong Agricultural University, Tai'an, 271000, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Dong</ForeName><Initials>D</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, 100193, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Xili</ForeName><Initials>X</Initials><AffiliationInfo><Affiliation>Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, 100193, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Tyler</LastName><ForeName>Brett M</ForeName><Initials>BM</Initials><AffiliationInfo><Affiliation>Center for Genome Research and Biocomputing and Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331, USA.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>04</Month><Day>06</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Environ Microbiol Rep</MedlineTA><NlmUniqueID>101499207</NlmUniqueID><ISSNLinking>1758-2229</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D006845">Hydrocarbons, Fluorinated</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011720">Pyrazoles</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011987">Receptors, Steroid</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C054899">oxysterol binding protein</NameOfSubstance></Chemical><Chemical><RegistryNumber>56498AIV8R</RegistryNumber><NameOfSubstance UI="C000592431">oxathiapiprolin</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D064113" MajorTopicYN="N">CRISPR-Cas Systems</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020043" MajorTopicYN="N">Genes, Essential</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006845" MajorTopicYN="N">Hydrocarbons, Fluorinated</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010838" MajorTopicYN="N">Phytophthora</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName><QualifierName UI="Q000472" MajorTopicYN="Y">pathogenicity</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011720" MajorTopicYN="N">Pyrazoles</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011987" MajorTopicYN="N">Receptors, Steroid</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017384" MajorTopicYN="N">Sequence Deletion</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014774" MajorTopicYN="N">Virulence</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2018</Year><Month>01</Month><Day>15</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2018</Year><Month>03</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>03</Month><Day>05</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2018</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2019</Year><Month>2</Month><Day>26</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>3</Month><Day>10</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">29521469</ArticleId><ArticleId IdType="doi">10.1111/1758-2229.12638</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Miao, Jianqiang" sort="Miao, Jianqiang" uniqKey="Miao J" first="Jianqiang" last="Miao">Jianqiang Miao</name></noRegion><name sortKey="Li, Xiuhuan" sort="Li, Xiuhuan" uniqKey="Li X" first="Xiuhuan" last="Li">Xiuhuan Li</name><name sortKey="Lin, Dong" sort="Lin, Dong" uniqKey="Lin D" first="Dong" last="Lin">Dong Lin</name><name sortKey="Liu, Xili" sort="Liu, Xili" uniqKey="Liu X" first="Xili" last="Liu">Xili Liu</name></country><country name="États-Unis"><noRegion><name sortKey="Tyler, Brett M" sort="Tyler, Brett M" uniqKey="Tyler B" first="Brett M" last="Tyler">Brett M. 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